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Assay:

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Id: 30059
Copied From: 30058
 
GENERAL INFORMATION
 
Authors: Megan Davey
Lab: Roslin Institute
Standard Protocol Id: Roslin
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: MICALL1_Probe_1_AS_DB
Gene Details
Gallus gallus Id: GG:989
Entity Detected: MICALL1
Gene Name: MICAL-like 1 [Gallus gallus]
Entrez Id: 418032
Ensembl Id: ENSGALG00000012265
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: ISA Brown
Stage: HH21
Stage Qualifier: Exact
Sex:
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-09-13
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 30
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: High
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: Somites, mesenchyme and limb mesenchyme.
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1842
  Name: somite
  Strength: Strong
  Pattern: Regional
  Direction:
  Note:
 
Annotation ID: TMP:221
  Name: body wall mesenchyme
  Strength: Not Detected
  Pattern: Homogeneous
  Direction:
  Note:
 
Annotation ID: TMP:2343
  Name: wing mesenchyme
  Strength: Not Detected
  Pattern: Homogeneous
  Direction:
  Note:
 
Annotation ID: TMP:788
  Name: hind limb mesenchyme
  Strength: Not Detected
  Pattern: Homogeneous
  Direction:
  Note:
 
 
MISCELLANEOUS
 
References:
 
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