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Assay:

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Id: 40561
Copied From:
 
GENERAL INFORMATION
 
Authors: Claire Anderson
Lab: University College London
Standard Protocol Id: ucl
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation: BULK UPDATE – 2012-01-17
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 40562 | 40563
Linked Data - Same Embryo:
 
PROBE
 
Probe: SOCS2_Probe_1_AS_CS
Gene Details
Gallus gallus Id: GG:630
Entity Detected: SOCS2
Gene Name: suppressor of cytokine signaling 2 [Gallus gallus]
Entrez Id: 395219
Ensembl Id: ENSGALG00000011295
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Brown Bovan Gold
Stage: HH09
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2011-10-21
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 8
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In:
Not Detected In:
Possibly Detected In:
 
ANNOTATIONS
 
Annotation ID: TMP:1277
  Name: neural plate
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note: posterior
 
Annotation ID: TMP:1545
  Name: presomitic mesoderm
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:909
  Name: intermediate mesoderm
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:1995
  Name: splanchnic lateral plate mesoderm
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:477
  Name: ectoderm
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
Annotation ID: TMP:142
  Name: anterior intestinal portal
  Strength: Moderate
  Pattern: Regional
  Direction: Unknown
  Note: endoderm
 
Annotation ID: TMP:1672
  Name: rhombomere
  Strength: Strong
  Pattern: Regional
  Direction: Unknown
  Note:
 
 
MISCELLANEOUS
 
References:
 
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