Submission Interface

Assay:

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Id: 40071
Copied From: 40061
 
GENERAL INFORMATION
 
Authors: Jackie Liu
Lab: University College London
Standard Protocol Id: ucl
Lab Book Reference:
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment:
Linked Data - Same Embryo:
 
PROBE
 
Probe: SHH_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:8012
Entity Detected: SHH
Gene Name: sonic hedgehog homolog (Drosophila) [Gallus gallus]
Entrez Id: 395615
Ensembl Id: ENSGALG00000006379
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: Rhode Island Red
Stage: HH03
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative:
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2010-08-16
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label: Alkaline Phosphatase
Detected Using: NBT+BCIP
Development Time: 12
Time Unit: Hours
 
IMAGING PROCEDURE
 
Image Technique: Photography
Clearing Agent:
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: photography
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: Hensen's node
Not Detected In: area opaca,primitive streak
Possibly Detected In:
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References:
 
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