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Assay:

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Id: 20224
Copied From: 20223
 
GENERAL INFORMATION
 
Authors: Gordana
Lab: University of Bath
Standard Protocol Id: Bath1.1
Lab Book Reference: 07-12_2009
Confidence: High
Status: Public
Notes For Interpretation: Small eye
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 20222|20223
Linked Data - Same Embryo:
 
PROBE
 
Probe: E2F7_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:570
Entity Detected: E2F7
Gene Name: E2F transcription factor 7 [Gallus gallus]
Entrez Id: 417868
Ensembl Id: ENSGALG00000010265
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH26
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-12-07
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label:
Detected Using: NBT/BCIP concentration 2x lower than in Bath1
Development Time: 35
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: limb mesenchyme
Not Detected In:
Possibly Detected In: spinal cord neurons
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References: Towers and Tickle, Gene Pattern Expressions, 2009
 
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