Submission Interface

Assay:

Back
Id: 20218
Copied From: 20217
 
GENERAL INFORMATION
 
Authors: Gordana
Lab: University of Bath
Standard Protocol Id: Bath1.1
Lab Book Reference: 07-12_2009
Confidence: High
Status: Public
Notes For Interpretation:
Linked Data - Replicated Experiment:
Linked Data - Same Experiment: 20216|20217
Linked Data - Same Embryo:
 
PROBE
 
Probe: E2F7_Probe_1_AS_CT
Gene Details
Gallus gallus Id: GG:570
Entity Detected: E2F7
Gene Name: E2F transcription factor 7 [Gallus gallus]
Entrez Id: 417868
Ensembl Id: ENSGALG00000010265
 
Chemistry: RNA
Label: Digoxigenin
 
SPECIMEN
 
Organism: Gallus gallus
Breed: White Leghorn
Stage: HH21
Stage Qualifier: Exact
Sex: Unspecified
Mutation: None (Wild-Type)
Mounting: Wholemount
Fixative: 4% PFA
Embedding Reagent:
 
HYBRIDIZATION PROCEDURE
 
Hybridization Date: 2009-12-07
Secondary: Anti-Digoxigenin Ab
Tertiary:
Quaternary:
Final Label:
Detected Using: NBT/BCIP concentration 2x lower than in Bath1
Development Time: 35
Time Unit: Minutes
 
IMAGING PROCEDURE
 
Image Technique: OPT
Clearing Agent: BABB
 
IMAGE FILES
 
Local Zip File or Comment:
Number of Image Sets: 1
Image Folder: bioptonics
Image Quality: Unknown
 
EXPRESSION PATTERN DESCRIPTION
 
Detected In: limb mesenchyme | first pharyngeal arch: mandibular process | first pharyngeal arch: maxillary process | 2nd pharyngeal arch
Not Detected In:
Possibly Detected In: neural tube | spinal cord neurons
 
ANNOTATIONS
 
 
MISCELLANEOUS
 
References: Towers and Tickle, Gene Pattern Expressions, 2009
 
Back